FastQCFastQC Report
Thu 27 Jul 2023
test1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenametest1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160948
Total Bases869.9 Mbp
Sequences flagged as poor quality0
Sequence length0-154373
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAA49630211.92761841772596No Hit
CCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAG43023110.339735079602052No Hit
AGCCGCTATGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA2356095.662387513614686No Hit
TGCCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAA2316805.567961916370981No Hit
CCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAG2094335.033300103726362No Hit
GGGGGATATGGCGAAATTGGTAGACGCTACGGACTTAATTGGATTGAGCC1889644.5413689380400815No Hit
AGCCGCTATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA1628673.914180133950244No Hit
AGGGGATATGGCGAAATTGGTAGACGCTACGGACTTAATTGGATTGAGCC751481.8060307410715057No Hit
GGGGGATATGGCGAAATTGGTAGACGCTACGGACTTGATTGGATTGAGCC719021.7280196724400305No Hit
AGCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA640141.5384474884088915No Hit
AGCCGCCATGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA624411.5006436033327022No Hit
GGGGGATATGGCGAAATCGGTAGACGCTACGGACTTAATTGGATTGAGCC621641.4939864665456044No Hit
AGCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTC533761.282784596202596No Hit
TGGGGATATGGCGAAATCGGTAGACGCTACGGACTTGATTGTATTGAGCC487111.1706707221527402No Hit
CCGCTATGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCTAGA466151.1202975860308757No Hit
AGGGGATATGGCGAAATTGGTAGACGCTACGGACTTGATTGGATTGAGCC464601.116572473388276No Hit
CCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAA396720.9534365726271994No Hit
CCGCTATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTAGA284870.6846276377402457No Hit
TGCCGCTATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA266700.6409597043750607No Hit
GCCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAA220050.5288458303252047No Hit
GGCCGCTATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA210480.5058462638802503No Hit
GGGATATGGCGAAATTGGTAGACGCTACGGACTTAATTGGATTGAGCCTT206220.49560821235929886No Hit
AGGGGATATGGCGAAATCGGTAGACGCTACGGACTTAATTGGATTGAGCC202800.48738893156078855No Hit
AGCCGCTATGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCGA198650.47741524287253767No Hit
CCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTCAAG186710.44871985903212436No Hit
ATGCCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTA180130.4329061550396689No Hit
TGCCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTCA179240.43076721939327284No Hit
AGGGGATATGGCGAAATTGGTAGACGCTGCGGACTTAATTGGATTGAGCC169460.4072629602677082No Hit
TGCCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTTGTGCTAA167420.4023602313703512No Hit
CCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAT164070.3943091814653776No Hit
AGGGGATATGGCGAAATTGGTAGACGCTACGGACTTGGTTGGATTGAGCC150870.36258564154130263No Hit
AGGGGATATGGCGGAATTGGTAGACGCTACGGACTTAATTGGATTGAGCC139970.33638968811914977No Hit
CCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTTGTGCTAAAG138920.33386622471609834No Hit
TGCCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTTGTGCTAA137130.32956432043851547No Hit
CGCCGCTATGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA135200.324925954373859No Hit
CCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTTGTGCTAAAG132150.31759589401261445No Hit
CCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTAGA125700.30209461882244143No Hit
CCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAT116810.28072929534327273No Hit
CCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTGAAG104130.250255470628328No Hit
CCGCCATGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCTAGA99980.2402817819400771No Hit
TGCCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTGA99150.23828704420242697No Hit
CCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAC98240.23610004258644907No Hit
AGCCGCTATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCGA96450.23179813830886617No Hit
GGGGGATATGGCGAAATTGGTAGACGCTACGGACTTAATTGAATTGAGCC95980.23066858802369075No Hit
GGGGGATATGGCGAAATTGGTAGACGCTACGGACTTAATTAAATTGAGCC84780.20375164505780893No Hit
AGGGGATATGGCGAAATCGGTAGACGCTACGGACTTGATTAAATTGAGCC79720.1915909547535802No Hit
TTCTAATATGGCAGATTATATGTAATGGATTTAAACCCCATTTATAAAGG78140.18779374315660757No Hit
CGCCGCTATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA76540.18394846559005304No Hit
GGGGGATATGGCGGAATTGGTAGACGCTACGGACTTAATTGGATTGAGCC69040.1659237269968286No Hit
TGCCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTTA68970.16575549610329185No Hit
GGGGGATATGGCGAAATAGGTAGACGCTACGGACTTAATTGGATTGAGCC67600.1624629771869295No Hit
TGGGGATATGGCGAAATTGGTAGACGCTACGGACTTAATTGGATTGAGCC67580.16241491121734758No Hit
CCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAC67430.1620544164454831No Hit
CCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCGAGA63950.15369093773822698No Hit
AGCCGCTATGGTGAAATAGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA63350.152248958650769No Hit
GGGGGATATGGCGAAATTGGTAGACGCTACGGACTTGATTGAATTGAGCC61550.14792302138839514No Hit
GGCCGCTATGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA61540.1478989884036042No Hit
CCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTTGTGCTCAAG60160.14458243650245087No Hit
ATGCCTTGAAGAGGACTCGAACCTCCACGCTCTTTAGCACGAGATTTTGA59910.14398161188267675No Hit
TGCCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTTGTGCTCA58500.14059296102715055No Hit
AGCCGCCATGGTGAAATTGGTAGACACGCTGTTCTTAGGAAGCAGTGCTA57270.13763690389786173No Hit
AGCCGCTATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTT56630.1360987928712399No Hit
ATGCCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTA48180.11579092072287374No Hit
AGCCGCCGTGGTGAAATCGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA45240.10872522319432976No Hit
CGCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGGAAGCAGTGCTA45010.10817246454413754No Hit
GGGATATGGCGGAATTGGTAGACGCTACGGACTTGATCGAATTGAGCCTT43190.10379846131218175No Hit
AGGGGATATGGCGAAATCGGTAGACGCTACGGACTTAATTGGCTTGAGCC42480.10209211939202316No Hit
CCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAA42470.1020680864072322No Hit
GCCTTGATGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAA42340.10175565760494965No Hit
GGGGGATATGGCGGAATCGGTAGACGCTACGGACTTAATTGGATTGAGCC42330.10173162462015868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)