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        Download the raw data used to create the plots in this report below:

        Note that additional data was saved in multiqc_PRJNA763023_report_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        To help with this, you can download publication details of the tools mentioned in this report:

        About MultiQC

        This report was generated using MultiQC, version 1.15

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2023-09-14, 14:25 PDT based on data in:

        General Statistics

        Showing 200/200 rows and 4/6 columns.
        Sample Name% Dups% GCMedian Read LengthM Seqs
        SRR16124168
        0.0%
        0%
        0 bp
        0.0
        SRR16124169
        26.2%
        49%
        300 bp
        21.5
        SRR16124170
        25.4%
        52%
        300 bp
        35.7
        SRR16124171
        9.4%
        48%
        300 bp
        1.0
        SRR16124172
        38.4%
        48%
        300 bp
        24.9
        SRR16124173
        33.7%
        49%
        300 bp
        24.5
        SRR16124174
        0.0%
        0%
        0 bp
        0.0
        SRR16124175
        48.7%
        46%
        300 bp
        33.7
        SRR16124176
        0.0%
        0%
        0 bp
        0.0
        SRR16124177
        29.8%
        47%
        300 bp
        36.5
        SRR16124178
        36.4%
        48%
        300 bp
        37.2
        SRR16124179
        40.9%
        47%
        300 bp
        34.5
        SRR16124180
        10.1%
        48%
        300 bp
        2.1
        SRR16124181
        23.4%
        50%
        300 bp
        34.8
        SRR16124182
        13.4%
        47%
        300 bp
        1.1
        SRR16124183
        30.5%
        49%
        300 bp
        36.8
        SRR16124184
        12.4%
        49%
        300 bp
        7.1
        SRR16124185
        0.0%
        0%
        0 bp
        0.0
        SRR16124186
        14.7%
        48%
        300 bp
        9.8
        SRR16124187
        8.1%
        48%
        300 bp
        1.2
        SRR16124188
        48.7%
        48%
        300 bp
        38.7
        SRR16124189
        9.7%
        51%
        300 bp
        18.6
        SRR16124190
        0.0%
        0%
        0 bp
        0.0
        SRR16124191
        0.0%
        0%
        0 bp
        0.0
        SRR16124192
        50.3%
        48%
        300 bp
        37.1
        SRR16124193
        20.1%
        46%
        300 bp
        11.1
        SRR16124194
        0.0%
        0%
        0 bp
        0.0
        SRR16124195
        8.7%
        51%
        300 bp
        8.5
        SRR16124196
        0.0%
        0%
        0 bp
        0.0
        SRR16124197
        15.3%
        50%
        300 bp
        17.5
        SRR16124198
        0.0%
        0%
        0 bp
        0.0
        SRR16124199
        11.1%
        49%
        300 bp
        10.6
        SRR16124200
        0.0%
        0%
        0 bp
        0.0
        SRR16124201
        48.8%
        49%
        300 bp
        36.7
        SRR16124202
        12.7%
        47%
        300 bp
        5.7
        SRR16124203
        16.5%
        49%
        300 bp
        10.0
        SRR16124204
        36.8%
        47%
        300 bp
        34.4
        SRR16124205
        38.8%
        52%
        300 bp
        35.6
        SRR16124206
        8.4%
        47%
        300 bp
        0.9
        SRR16124207
        0.0%
        0%
        0 bp
        0.0
        SRR16124208
        0.0%
        0%
        0 bp
        0.0
        SRR16124209
        0.0%
        0%
        0 bp
        0.0
        SRR16124210
        0.0%
        0%
        0 bp
        0.0
        SRR16124211
        33.4%
        48%
        300 bp
        30.2
        SRR16124212
        0.0%
        0%
        0 bp
        0.0
        SRR16124213
        29.8%
        50%
        300 bp
        47.8
        SRR16124214
        6.1%
        48%
        300 bp
        3.1
        SRR16124215
        39.4%
        48%
        300 bp
        20.4
        SRR16124216
        14.7%
        46%
        300 bp
        4.2
        SRR16124217
        31.7%
        46%
        300 bp
        30.3
        SRR16124218
        8.4%
        49%
        300 bp
        3.2
        SRR16124219
        0.0%
        0%
        0 bp
        0.0
        SRR16124220
        0.0%
        0%
        0 bp
        0.0
        SRR16124221
        30.3%
        48%
        300 bp
        34.8
        SRR16124222
        0.0%
        0%
        0 bp
        0.0
        SRR16124223
        31.6%
        51%
        300 bp
        36.9
        SRR16124224
        23.8%
        48%
        300 bp
        14.9
        SRR16124225
        20.6%
        48%
        300 bp
        25.9
        SRR16124226
        46.8%
        51%
        300 bp
        22.1
        SRR16124227
        0.0%
        0%
        0 bp
        0.0
        SRR16124228
        25.2%
        48%
        300 bp
        33.4
        SRR16124229
        38.2%
        48%
        300 bp
        34.6
        SRR16124230
        50.5%
        45%
        300 bp
        36.6
        SRR16124231
        44.7%
        47%
        300 bp
        20.7
        SRR16124232
        0.0%
        0%
        0 bp
        0.0
        SRR16124233
        46.5%
        47%
        300 bp
        41.7
        SRR16124234
        0.0%
        0%
        0 bp
        0.0
        SRR16124235
        0.0%
        0%
        0 bp
        0.0
        SRR16124236
        10.8%
        50%
        300 bp
        6.8
        SRR16124237
        62.8%
        47%
        300 bp
        35.1
        SRR16124238
        28.5%
        49%
        300 bp
        36.1
        SRR16124239
        30.1%
        49%
        300 bp
        33.9
        SRR16124240
        21.9%
        49%
        300 bp
        20.4
        SRR16124241
        0.0%
        0%
        0 bp
        0.0
        SRR16124242
        16.4%
        49%
        300 bp
        4.3
        SRR16124243
        22.5%
        48%
        300 bp
        17.1
        SRR16124244
        0.0%
        0%
        0 bp
        0.0
        SRR16124245
        32.6%
        47%
        300 bp
        37.6
        SRR16124246
        41.1%
        47%
        300 bp
        35.8
        SRR16124247
        0.0%
        0%
        0 bp
        0.0
        SRR16124248
        40.5%
        44%
        300 bp
        35.2
        SRR16124249
        9.9%
        46%
        300 bp
        1.0
        SRR16124250
        25.7%
        49%
        300 bp
        35.3
        SRR16124251
        42.1%
        46%
        300 bp
        37.3
        SRR16124252
        0.0%
        0%
        0 bp
        0.0
        SRR16124253
        13.0%
        49%
        300 bp
        10.1
        SRR16124254
        0.0%
        0%
        0 bp
        0.0
        SRR16124255
        32.2%
        45%
        300 bp
        11.2
        SRR16124256
        21.6%
        48%
        300 bp
        7.1
        SRR16124257
        13.3%
        46%
        300 bp
        3.3
        SRR16124258
        33.8%
        48%
        300 bp
        39.1
        SRR16124259
        23.6%
        48%
        300 bp
        42.2
        SRR16124260
        5.4%
        49%
        300 bp
        1.3
        SRR16124261
        6.1%
        48%
        300 bp
        1.4
        SRR16124262
        8.5%
        49%
        300 bp
        3.0
        SRR16124263
        12.4%
        50%
        300 bp
        17.6
        SRR16124264
        5.8%
        47%
        300 bp
        1.2
        SRR16124265
        43.1%
        48%
        300 bp
        42.5
        SRR16124266
        52.1%
        48%
        300 bp
        22.7
        SRR16124267
        30.3%
        51%
        300 bp
        58.6
        SRR16124268
        0.0%
        0%
        0 bp
        0.0
        SRR16124269
        31.2%
        45%
        300 bp
        34.5
        SRR16124270
        0.0%
        0%
        0 bp
        0.0
        SRR16124271
        9.0%
        46%
        300 bp
        2.2
        SRR16124272
        25.2%
        47%
        300 bp
        21.3
        SRR16124273
        33.6%
        48%
        300 bp
        10.2
        SRR16124274
        0.0%
        0%
        0 bp
        0.0
        SRR16124275
        7.2%
        47%
        300 bp
        4.9
        SRR16124276
        0.0%
        0%
        0 bp
        0.0
        SRR16124277
        19.2%
        50%
        300 bp
        6.6
        SRR16124278
        10.3%
        45%
        300 bp
        15.9
        SRR16124279
        0.0%
        0%
        0 bp
        0.0
        SRR16124280
        0.0%
        0%
        0 bp
        0.0
        SRR16124281
        0.0%
        0%
        0 bp
        0.0
        SRR16124282
        0.0%
        0%
        0 bp
        0.0
        SRR16124283
        13.2%
        47%
        300 bp
        6.5
        SRR16124284
        19.1%
        46%
        300 bp
        17.1
        SRR16124285
        0.0%
        0%
        0 bp
        0.0
        SRR16124286
        34.5%
        51%
        300 bp
        38.2
        SRR16124287
        19.8%
        47%
        300 bp
        7.2
        SRR16124288
        22.7%
        50%
        300 bp
        35.7
        SRR16124289
        0.0%
        0%
        0 bp
        0.0
        SRR16124290
        33.5%
        47%
        300 bp
        31.0
        SRR16124291
        6.7%
        47%
        300 bp
        2.2
        SRR16124292
        14.1%
        54%
        300 bp
        21.3
        SRR16124293
        19.1%
        46%
        300 bp
        14.4
        SRR16124294
        19.9%
        48%
        300 bp
        38.2
        SRR16124295
        10.3%
        47%
        300 bp
        3.6
        SRR16124296
        42.4%
        46%
        300 bp
        40.5
        SRR16124297
        0.0%
        0%
        0 bp
        0.0
        SRR16124298
        24.0%
        50%
        300 bp
        40.6
        SRR16124299
        34.6%
        51%
        300 bp
        6.6
        SRR16124300
        14.7%
        47%
        300 bp
        11.8
        SRR16124301
        6.4%
        48%
        300 bp
        3.3
        SRR16124302
        0.0%
        0%
        0 bp
        0.0
        SRR16124303
        0.0%
        0%
        0 bp
        0.0
        SRR16124304
        0.0%
        0%
        0 bp
        0.0
        SRR16124305
        6.2%
        47%
        300 bp
        1.1
        SRR16124306
        33.3%
        47%
        300 bp
        32.2
        SRR16124307
        18.0%
        47%
        300 bp
        28.0
        SRR16124308
        8.4%
        47%
        300 bp
        2.4
        SRR16124309
        0.0%
        0%
        0 bp
        0.0
        SRR16124310
        9.7%
        45%
        300 bp
        1.2
        SRR16124311
        30.6%
        50%
        300 bp
        30.4
        SRR16124312
        0.0%
        0%
        0 bp
        0.0
        SRR16124313
        25.5%
        47%
        300 bp
        27.5
        SRR16124314
        32.2%
        50%
        300 bp
        12.6
        SRR16124315
        0.0%
        0%
        0 bp
        0.0
        SRR16124316
        31.1%
        46%
        300 bp
        38.9
        SRR16124317
        20.4%
        49%
        300 bp
        41.9
        SRR16124318
        33.4%
        47%
        300 bp
        35.7
        SRR16124319
        27.1%
        48%
        300 bp
        33.3
        SRR16124320
        14.7%
        45%
        300 bp
        35.9
        SRR16124321
        14.4%
        49%
        300 bp
        12.7
        SRR16124322
        24.8%
        46%
        300 bp
        20.0
        SRR16124323
        18.9%
        48%
        300 bp
        15.3
        SRR16124324
        0.0%
        0%
        0 bp
        0.0
        SRR16124325
        17.7%
        46%
        300 bp
        8.7
        SRR16124326
        25.5%
        48%
        300 bp
        34.9
        SRR16124327
        36.4%
        45%
        300 bp
        31.9
        SRR16124328
        10.9%
        47%
        300 bp
        3.4
        SRR16124329
        13.2%
        50%
        300 bp
        9.5
        SRR16124330
        0.0%
        0%
        0 bp
        0.0
        SRR16124331
        8.7%
        46%
        300 bp
        0.9
        SRR16124332
        0.0%
        0%
        0 bp
        0.0
        SRR16124333
        11.6%
        50%
        300 bp
        7.4
        SRR16124334
        0.0%
        0%
        0 bp
        0.0
        SRR16124335
        23.3%
        50%
        300 bp
        38.8
        SRR16124336
        0.0%
        0%
        0 bp
        0.0
        SRR16124337
        19.0%
        47%
        300 bp
        20.9
        SRR16124338
        0.0%
        0%
        0 bp
        0.0
        SRR16124339
        18.0%
        45%
        300 bp
        13.2
        SRR16124340
        37.3%
        45%
        300 bp
        28.6
        SRR16124341
        0.0%
        0%
        0 bp
        0.0
        SRR16124342
        0.0%
        0%
        0 bp
        0.0
        SRR16124343
        23.9%
        47%
        300 bp
        42.7
        SRR16124344
        0.0%
        0%
        0 bp
        0.0
        SRR16124345
        0.0%
        0%
        0 bp
        0.0
        SRR16124346
        12.3%
        46%
        300 bp
        45.8
        SRR16124347
        0.0%
        0%
        0 bp
        0.0
        SRR16124348
        0.0%
        0%
        0 bp
        0.0
        SRR16124349
        9.1%
        53%
        300 bp
        3.1
        SRR16124350
        9.6%
        46%
        300 bp
        5.5
        SRR16124351
        18.0%
        47%
        300 bp
        13.7
        SRR16124352
        17.9%
        50%
        300 bp
        37.7
        SRR16124353
        0.0%
        0%
        0 bp
        0.0
        SRR16124354
        13.5%
        48%
        300 bp
        9.8
        SRR16124355
        3.9%
        51%
        300 bp
        1.3
        SRR16124356
        0.0%
        0%
        0 bp
        0.0
        SRR16124357
        34.8%
        51%
        300 bp
        32.2
        SRR16124358
        0.0%
        0%
        0 bp
        0.0
        SRR16124359
        0.0%
        0%
        0 bp
        0.0
        SRR16124360
        31.1%
        49%
        300 bp
        41.1
        SRR16124361
        22.3%
        49%
        300 bp
        39.9
        SRR16124362
        22.3%
        46%
        300 bp
        41.1
        SRR16124363
        31.2%
        43%
        300 bp
        32.9
        SRR16124364
        0.0%
        0%
        0 bp
        0.0
        SRR16124365
        0.0%
        0%
        0 bp
        0.0
        SRR16124366
        19.9%
        48%
        300 bp
        37.6
        SRR16124367
        7.9%
        56%
        300 bp
        1.1

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        loading..

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        loading..

        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help

        loading..

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        200 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

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        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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        MultiQC v1.15 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.