SUMMARISING RUN PARAMETERS ========================== Input filename: Raw_Reads/H3K4me3_TwoCell.fq.gz Trimming mode: single-end Trim Galore version: 0.4.1 Cutadapt version: 1.10 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length before a sequence gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 1.10 with Python 2.7.12 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Raw_Reads/H3K4me3_TwoCell.fq.gz Trimming 1 adapter with at most 10.0% errors in single-end mode ... cutadapt: error: Line 3 in FASTQ file is expected to start with '+', but found ';' gzip: stdout: Broken pipe