SUMMARISING RUN PARAMETERS ========================== Input filename: Samples/H3K4me3_Liver_A/raw_reads.fq.gz Trimming mode: single-end Trim Galore version: 0.4.0 Cutadapt version: 1.12 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length before a sequence gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 1.12 with Python 2.7.6 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC Samples/H3K4me3_Liver_A/raw_reads.fq.gz Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 457.37 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 17,143,420 Reads with adapters: 5,846,106 (34.1%) Reads written (passing filters): 17,143,420 (100.0%) Total basepairs processed: 874,314,420 bp Quality-trimmed: 634,758 bp (0.1%) Total written (filtered): 865,110,481 bp (98.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 5846106 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 32.9% C: 30.4% G: 18.2% T: 18.3% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 4210797 4285855.0 0 4210797 2 1193618 1071463.8 0 1193618 3 342436 267865.9 0 342436 4 69571 66966.5 0 69571 5 13276 16741.6 0 13276 6 1028 4185.4 0 1028 7 479 1046.4 0 479 8 252 261.6 0 252 9 380 65.4 0 190 190 10 544 16.3 1 211 333 11 320 4.1 1 160 160 12 218 1.0 1 142 76 13 234 0.3 1 171 63 14 186 0.3 1 134 52 15 228 0.3 1 168 60 16 185 0.3 1 143 42 17 230 0.3 1 168 62 18 232 0.3 1 173 59 19 168 0.3 1 133 35 20 179 0.3 1 140 39 21 151 0.3 1 113 38 22 168 0.3 1 133 35 23 157 0.3 1 116 41 24 145 0.3 1 107 38 25 155 0.3 1 113 42 26 120 0.3 1 87 33 27 126 0.3 1 98 28 28 128 0.3 1 100 28 29 98 0.3 1 78 20 30 79 0.3 1 69 10 31 58 0.3 1 43 15 32 72 0.3 1 55 17 33 60 0.3 1 43 17 34 73 0.3 1 56 17 35 46 0.3 1 26 20 36 43 0.3 1 24 19 37 36 0.3 1 23 13 38 32 0.3 1 21 11 39 29 0.3 1 22 7 40 11 0.3 1 3 8 41 19 0.3 1 6 13 42 23 0.3 1 5 18 43 10 0.3 1 3 7 44 8 0.3 1 1 7 45 10 0.3 1 0 10 46 7 0.3 1 0 7 47 18 0.3 1 0 18 48 16 0.3 1 0 16 49 24 0.3 1 0 24 50 95 0.3 1 0 95 51 9528 0.3 1 4 9524 RUN STATISTICS FOR INPUT FILE: Samples/H3K4me3_Liver_A/raw_reads.fq.gz ============================================= 17143420 sequences processed in total Sequences removed because they became shorter than the length cutoff of 20 bp: 10425 (0.1%)